Fig. 6

a Murine CH12F3 cells were subjected to HDACi treatment at the given concentrations for 48 h prior to western analysis of UNG1/2 expression. b Representative flow cytograms illustrating dose-dependent reduction in IgM to IgA class switching in CH12F3 cells with induction treatment and addition of HDACi as indicated. c Quantification of genomic uracil in HEK293, HeLa, Jurkat and SUDHL5 cells after 48 h treatment with 2 µM SAHA or 5 µM MS-275 for 48 h. Red dashed line indicates dU level in UNG-treated DNA control (detection limit)