Fig. 4

LTBP1 promoted EMT and chemoresistant in ESCC cells. a Silencing LTBP1 inhibited hallmarks of the EMT, including loss of N-cadherin/vimentin and accumulation of E-cadherin in ESCC cells. b ESCC treated with si-NC or si-LTBP1 for 24 h, and treated with/without 20 ng/ml TGFβ for an additional 48 h. Western blot analysis of EMT markers in ESCC cells. c Morphological changes were observed under distinct treatment conditions. e BCL2 expression decreased and BAX expression increased over time in si-LTBP1 cells treated with 5-FU for 48 h. d, f Inhibition rate were detected by CCK8 assays and apoptosis rate was detected by flow cytometric analysis of ESCC cells after treatment with 5-FU for 48 h. Each experiment was performed in triplicate. Paired-samples t-test, *p < 0.05