Fig. 4

Study of IL-6 effect on ciliary beating. Comparison of ciliary beating parameters between IL-6 groups at increased concentrations (IL6-1, IL6-10, IL6-100) (1 ng/ml, 10 ng/ml, 100 ng/ml respectively)) and control group after 21 days of stimulation by IL-6 in culture medium of HNEC (n = 21 controls wells and n = 21 wells with IL6-100 and n = 21 wells with IL6-10 and and n = 21 wells with IL6-1) (paired analysis): comparison of mean ciliary beat frequency (CBF) (in Herz) (a), mean relative ciliary density (b), mean cilia length (c), mean metachronal wavelength (LOOM) (in μm) (d), mean efficiency index of beating (in mPa) (e). f, g Focus of significant results of paired analysis: variation of ciliary beat frequency (CBF) between each well with IL-6 stimulation at 100 ng/ml and its respective control well (n = 21) (f) and focus on variation of metachronal wavelength (LOOM) between each well with IL-6 stimulation at 100 ng/ml and its respective control well (n = 21) (g)