Fig. 3

Changes in the acetylation pattern of lysines 5, 8, 12 and 16 during healing. a Schematic representation of the healing phase at day 4 shows the presence of normal epithelium, proliferative epithelium, and migratory epithelial tongue. Quantification of the percentage of epithelial cells positive for acetyl histone H4 at lysines 5, 8, 12, and 16 by day 4 after wound (n = 8 mice). Note deacetylation of histone H4 at lysines 5, 8, and 16, while lysine 12 is found hyperacetylated during the migration and proliferation phases (*p < 0.05, ***p < 0.001, ****p < 0.0001) (data represented as mean ± SEM). b Schematic representation of wound at day 9 depicts a complete wound closure. Quantification of the acetylation levels of histone H4 at lysines 5, 8, 12, and 16 shows overall deacetylation of lysines 5, 8, and 12, while lysine 16 is observed upregulated exclusively at the closed wound site (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) (n = 6 mice; data represented as mean ± SEM)