Fig. 2

Tissue injury triggers broad deacetylation of histone H4 in epithelial cells. a Schematic representation of nucleosomes formed by histones with emphasis on histone H4 and its 4 acetylation sites. b Schematic representation of the latent phase of the wound, which is characterized by the absence of epithelial migration. c Representative images of immunofluorescence staining for histone H4 acetylated at lysine 5 (H4K5_green), lysine 8 (H4K8_green), lysine 12 (H4K12_green), and lysine 16 (H4K16_red), as well as Alexa 488 (green), Alexa 568 (red), and Hoechst 33342 (counterstain). Dashed line delineates the epidermis. d Quantification of positive epithelial cells (%) stained for acetyl histone H4 at lysines 5, 8, 12, and 16 (**p < 0.01, ***p < 0.001, ****p < 0.0001) (n = 8 mice; data represented as mean ± SEM)