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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: High-intensity interval training in allogeneic adoptive T-cell immunotherapy – a big HIT?

Fig. 4

Effects. of a single continuous and interval exercise on the activation capacity of functional IFN-γ+ antigen-specific T cells. Peripheral blood samples of healthy donors (n = 6) were analysed before, 1 h after and 24 h after a single 30 min interval (HIT) using IFN-γ Cytokine Secretion Assay (CSA). Isolated PBMCs were stimulated overnight with a CMV-, b EBV- and c AdV-specific peptide pools (CMV pp65, CMV IE1, EBV EBNA1, EBV Consensus, AdV5 Hexon and AdV5 Penton). Negative control was carried out by using unstimulated PBMCs. The relevant cell fractions before and after enrichment were used for a detailed flow cytometric analysis of IFN-γ+ T-cell subsets. Results are shown as fold changes of the frequency of CD3+/IFN-γ+, CD8+/IFN-γ+ and CD4+/IFN-γ+ cells after subtracting those of the respective negative control. CMV and AdV results are displayed as mean ± SD

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