Fig. 2
From: High-intensity interval training in allogeneic adoptive T-cell immunotherapy – a big HIT?
Impact of a single continuous and interval exercise on antigen-specific T-cell responses. Peripheral blood samples of healthy donors (n = 12) were analysed at different time points before and after a single 30 min continuous (CONT) or interval (HIT) exercise (before, directly after, 1 h after and 24 h after exercise). Isolated PBMCs were stimulated overnight with a CMV-, b EBV- and c AdV-specific peptide pools (CMV pp65, CMV IE1, EBV EBNA1, EBV Consensus, AdV5 Hexon and AdV5 Penton) and frequencies of functional-active virus-specific T cells were determined by IFN-γ EliSpot assay. Negative and positive controls were carried out by using either medium without stimuli or 1 μg/ml staphylococcal enterotoxins B (SEB). Results are indicated as the number of spots per 2.5 × 105 cells/well (spw) and spots per 1000 CD3+ T cells, respectively after substracting the number of spw of the respective negative control. Positive controls showed a positive result for all analysed samples detected as TNTC (too numerous to count, TNTC ≥ 1000 spw). Results are displayed as mean ± SD. Asterisks indicate statistically significant differences between levels of induced cytokine responses (*p < 0.05)