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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Upregulation of microRNA-328-3p by hepatitis B virus contributes to THLE-2 cell injury by downregulating FOXO4

Fig. 6

FOXO4 is a target for miR-328-3p and attenuates cell injury under LPS and HBV stimulation. a Results of luciferase activity assay showed that 3′UTR of FOXO4 was directly targeted by miR-328-3p. **P < 0.01 vs. mimic NC + FOXO4 WT. b Western blot analysis of protein expression of FOXO4, IκB-α, p65, and p-p65 in THLE-2 cells transfected with miR-328-3p mimic, miR-328-3p inhibitor, or corresponding controls. *P < 0.05 vs. the mimic NC group, #P < 0.05 vs. the inhibitor NC group. c Cell proliferation detectecd by MTT assay, d cell apoptosis rate detected by flow cytometry, and e levels of TNF-α, IL-6, IL-8, IL-12, and IL-18 detected by ELISA in THLE-2 cells transfected with pcDNA3.1-FOXO4, si-FOXO4, or corresponding controls, under LPS (1 μg/mL, 24 h) and HBV stimulation. Values are presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01 vs. LPS + HBV + Vector, #P < 0.05, ##P < 0.01 vs. LPS + HBV + si-Ctrl

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