Fig. 4

Anti-P-selectin mAb treatment ameliorated renal oxidative stress in MRL/lpr mice. a Representative immunohistochemistry staining micrographs of gp91phox in the C57BL/6, MRL/lpr, saline, and anti-P-selectin groups (magnification of ×400). b Western blot analysis was used to assess altered gp91phox and p22phox expression in renal tissues from MRL/lpr mice. β-actin was used as a loading control. c The quantification of the average band intensity for gp91phox and p22phox in b. The values of expression in the control group were set as 1. d The expression of gp91phox and p22phox in the renal tissues of saline or anti-P-selectin mAb–treated MRL/lpr mice (the saline and anti-P-selectin groups) was determined by western blot analysis. e Densitometric analyses of the western blots in d. The relative intensities of the bands were normalized to the intensities of the respective β-actin signal, and the value of the saline group was set as 1. The results are presented as the mean ± SD. *P < 0.05 between the two indicated groups, as analyzed by Student’s t test (n = 6–9 for each group)