Fig. 3

Anti-P-selectin mAb treatment ameliorated renal hypoxia in MRL/lpr mice. a Representative hypoxia probe in situ staining micrographs of by hypoxyprobe™-1 (magnification of ×400). b Immunohistochemistry staining of HIF-1α in the glomerulus and tubular interstitium (magnification of ×400). c Western blot analysis was used to assess altered HIF-1α expression in renal tissues from MRL/lpr mice. β-actin was used as a loading control. d The quantification of the average band intensity for HIF-1α in c. The values of expression in the control group were set as 1. e The expression of HIF-1α in the renal tissues of saline or anti-P-selectin mAb–treated MRL/lpr mice (the saline and anti-P-selectin groups) was determined by western blot analysis. f Densitometric analyses of the western blots in e. The relative intensities of the bands were normalized to the intensities of the respective β-actin signal, and the value of the saline group was set as 1. The results are presented as the mean ± SD. *P < 0.05 between the two indicated groups, as analyzed by Student’s t test (n = 6–9 for each group). g Peritubular capillary count (PTC) in the kidney of each group. ***P < 0.001 as analyzed by ANOVA followed by Tukey’s multiple comparisons test. h Immunohistochemistry staining of CD31 for PTC counting in the glomerulus and tubular interstitium (magnification of ×400)