Fig. 1

Anti-P-selectin mAb treatment neutralized the increased P-selectin expression in lupus nephritis. a Representative immunostaining micrographs of P-selectin show increased P-selectin expression in the glomeruli and tubulointerstitium of MRL/lpr mice and the neutralization of P-selectin with anti-P-selectin mAb (magnification of ×400). b Western blot analysis was used to assess altered P-selectin expression in renal tissues from MRL/lpr mice. β-actin was used as a loading control. c The quantification of the average band intensity for P-selectin in b. The values of expression in the control group were set as 1. d The expression of P-selectin in the renal tissues of saline or anti-P-selectin mAb–treated MRL/lpr mice (the saline and anti-P-selectin groups) was determined by western blot analysis. e Densitometric analyses of the western blots in (D). The relative intensities of the bands were normalized to the intensities of the respective β-actin signal, and the value of the saline group was set as 1. The results are presented as the mean ± SD. *P < 0.05 between the two indicated groups, as analyzed by Student’s t test (n = 6–9 for each group)