Fig. 3

In the trans-well assay, microglia treated with lipopolysaccharide (LPS) were cultured in the upper chamber, and NSCs cultured in the lower chamber were treated with leukosomes or EPS-Leuko. a EdU cell proliferation assay showed the number of proliferating NSCs in different groups. b Annexin V-PI staining was used to determine apoptotic NSCs. The proportion of apoptotic cells was determined by flow cytometry. c Images of quantification showing the proliferation numbers of NSCs and apoptosis rate of NSCs in different groups. Scale bar, 100 μm (n = 8 per group). Values represent the mean ± SD, * and ^#P < 0.05, ** and ^##P < 0.01