Table 1 Primers used for RT-PCR
Gene | Primer sequences | Product size (bp) | GenBank accession no. | Species |
|---|---|---|---|---|
IL-6 | F:GAGTCACAGAAGGAGTGGCTAAGGA | 106 | NM_031168.1 | Mouse |
R:CGCACTAGGTTTGCCGAGTAGATCT | ||||
TNF-α | F:GCATGGTGGTGGTTGTTTCTGACGAT | 99 | NM_010851.2 | Mouse |
R:GCTTCTGTTGGACACCTGGAGACA | ||||
IL-1β | F:CTCGTGCTGTCGGACCCAT | 343 | NM_008361.4 | Mouse |
R:CAGGCTTGTGCTCTGCTTGTGA | ||||
Zo-1 | F:TCATCCCAAATAAGAACAGAGC | 198 | XM_006540786.1 | Mouse |
R:GAAGAACAACCCTTTCATAAGC | ||||
Occludin | F:AAGCAAGTGAAGGGATCTGC | 204 | NM_001205255.1 | Mouse |
R:GGGGTTATGGTCCAAAGTCA | ||||
GAPDH | F:CAACGGCACAGTCAAGGCTGAGA | 112 | NM_017008.3 | Mouse |
R:CTCAGCACCAGCATCACCCCAT | ||||
Zo-1 | F:AGAGGAAGCTGTGGGTAACG | 320 | NM_001301025.1 | Human |
R:TCCTTCAGCTGGTCCTCCTT | ||||
Occludin | F:CTCCCTGGCACCGTTGG | 548 | NM_001205254.1 | Human |
R:GGCCAACATGAAGCCCTTTG | ||||
β-Actin | F:CTCGCCTTTGCCGATCC | 258 | NM_001101.3 | Human |
R:GGGGTACTTCAGGGTGAGGA |