Fig. 8

Functional effects of HNF4A on ABAT and ALDH6A1 expression. a HEK293T and ACHN cells were transiently transfected with pHNF4A-Flag or a control vector for 24 h. The mRNA levels of HNF4A, ABAT and ALDH6A1 were detected by qRT-PCR. The experiments were repeated three times. The p-value was measured using Student’s t-test; *p < 0.05, compared with the control cells. b HEK293T and ACHN cells were transiently transfected with pHNF4A-Flag or a control vector for 24 h. The protein levels of HNF4A-Flag, ABAT, and ALDH6A1 were detected by Western blotting. The experiments were repeated three times. c ChIP assay was used to analyze HNF4A binding to the two regions of ABAT and ALDH6A1 promoter in HEK293T cells. The p-value was measured using Student’s t-test; *p < 0.05, compared with IgG control. The experiments were repeated three times. d HEK293T cells were transiently transfected with pRL-TK and pGL3-Basic-control, pGL3-ABAT-promoter or pGL3-ALDH6A1-promoter and together with the empty vector or HNF4A expression vector for 24 h. Cell lysates were analyzed for luciferase activity. The experiments were repeated three times. The p-value was measured using Student’s t-test; *p < 0.05, compared with that of the empty-vector-transfected control cells