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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Secretory clusterin promotes hepatocellular carcinoma progression by facilitating cancer stem cell properties via AKT/GSK-3β/β-catenin axis

Fig. 4

sCLU promoted the activity of β-catenin by AKT/GSK-3β signaling. a β-catenin expression in attached cells and spheroids was detected by western blotting. b CTNNB1 mRNA expression in attached cells and spheroids was detected by RT-qPCR. c sCLU and β-catenin mRNA expressions in HCC cell lines were detected by RT-qPCR. d Immunohistochemistry was performed to analyze the expression of sCLU and β-catenin in xenograft tumors. e The expressions of β-catenin in sCLU-repressed HCCLM3 cells or sCLU-overexpressed HepG2 cells were determined by immunofluorescence assay. f The key proteins of the AKT/GSK-3β/β-catenin axis in sCLU-repressed HCCLM3 cells were measured by western blotting. g Relative protein intensities of F were detected by Image J software. h The key proteins of the AKT/GSK-3β/β-catenin signaling pathway in sCLU-overexpressed HepG2 cells were measured by western blotting, with or without the treatment of LY294002. I Relative protein intensities in H were detected by Image J software. GAPDH was used as a loading control. **P < 0.01

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