Fig. 5
From: Suppression of p66Shc prevents hyperandrogenism-induced ovarian oxidative stress and fibrosis
Dihydrotestosterone promotes p66Shc phosphorylation resulting in mitochondrial dysfunction. Granulosa cells from naïve rats were treated with DHT, H2O2 or SOD. a p-p66Shc expression (Alexa Fluor 488) and mitochondria (MitoTracker Red) were revealed by confocal microscopy. b Quantification of the p-p66Shc fluorescence intensity is shown. c Quantification of MitoTrack Red intensity is shown. d Mitochondrial membrane potential was analyzed by the ratio of JC-1 monomers/polymers (60×). e Quantitative analysis of the ratios of the red to green fluorescence. Images are representative of three independent experiments with similar results. Data are shown as the mean ± SEM. *p ≤ 0.05. DHT, dihydrotestosterone; H2O2, hydrogen peroxide; SOD, superoxide dismutase; p-p66Shc, phosphorylated 66-kDa Src homology 2 domain-containing protein