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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Suppression of p66Shc prevents hyperandrogenism-induced ovarian oxidative stress and fibrosis

Fig. 3

Resveratrol inhibits p66Shc phosphorylation and fibrotic factors activation in rat ovaries. Rats received DHEA for the induction of polycystic ovarian syndrome, together with or without resveratrol treatment. a Sirt1, α-SMA, collagen I A1, CTGF, and p-p66Shc expression in rat ovaries (60×) was analyzed by double immunofluorescence staining. Images are representative of three independent experiments with similar results. b The expression of fibrotic factors and p-p66Shc in ovaries was assessed by western blot. The panel on the right shows the quantitative analysis. c The mRNA levels of AR was analyzed by real-time PCR. d Relative expression of p66Shc and fibrotic factors in rat ovaries was determined by real-time PCR. n = 7 in each group. Three independent experiments were performed with similar results. Data are shown as mean ± SD & mean ± SEM. *p ≤ 0.05, **p ≤ 0.01 vs. Blank; #p ≤ 0.05, ##p ≤ 0.01 vs. DHEA treatment. DHEA dehydroepiandrosterone, AR androgen receptor, CTGF connective tissue growth factor, Sirt1 sirtuin 1, p-p66Shc phosphorylated 66-kDa Src homology 2 domain-containing protein

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