Gene | Astrocyte vs. progenitor | Progenitor vs. stem cell | Astrocyte vs. stem cell |
|---|
Gfap | N.S. | ** | N.S. |
Hopx | N.S. | **** | ** |
Fabp7 | ** | N.S. | * |
Grin2c | **** | N.S. | *** |
Sox9 | F.C. | **** | F.C. |
Neurod1 | F.C. | F.C. | F.C. |
Dcx | F.C. | F.C. | F.C. |
Id4 | F.C. | *** | F.C. |
Pcna | F.C. | N.S. | F.C. |
Mcm2 | F.C. | F.C. | F.C. |
Ascl1 | F.C. | F.C. | F.C. |
Eomes | F.C. | F.C. | F.C. |
Nes | F.C. | F.C. | F.C. |
Neurog2 | F.C. | F.C. | F.C. |
- Genes known to be differentially expressed in murine astrocytes, stem cells and progenitors. lcRNA-seq data were preprocessed using CLEAR prior to between-group comparisons using DESeq2. Differential gene expression analysis evaluates the effectiveness of staining and FACS strategies in enriching these three cell types. The bisecting line indicates the border between transcripts which pass CLEAR in all samples and those that do not pass CLEAR in all samples. Each comparison was processed using DESeq2 and the significance of the comparison is given. q: FDR-corrected p-values; N.S. (Not Significant), F.C. (Failed CLEAR), * (q ≤ 0.05), ** (q ≤ 0.01), *** (q ≤ 0.001), **** (q ≤ 0.0001)
