Fig. 2

ZEB2 is directly targeted by miR-124 and miR-203. a Relative expression levels of ZEB2, miR-124 and miR-203 expression levels in renal cancer cell line (786-O) and normal immortalized renal cell line (HK2) as assessed by qRT-PCR; b schematic representation of the seed sequence of miR-124 and miR-203 and their putative binding sites in ZEB2 3′-UTR; c result of Luciferase assay in 786-O cell lines cotransfected with the ZEB2 wild type 3′UTR or ZEB2 mutated 3′UTR reporter plasmids and either miR-124/miR-203 mimics or scrambled miRNA mimics. The result is given in relation to the control luciferase activity. Data are calculated as mean ± SD of triplicates. d Effect of miRNA restoration on ZEB2 expression determined by qRT-PCR. miR-124 and/or miR-203 were transfected in 786-O cell lines (*p < 0.05; **p < 0.01; ns not significant)