Fig. 3
From: Lycopene and bone: an in vitro investigation and a pilot prospective clinical study
Lycopene increases mRNA, protein and activity of Alkaline Phosphatase on Saos-2 cells Semi-confluent cultures of human osteoblast-like cells (Saos-2) were incubated with lycopene 5 and 10 µM or vehicle THF in serum free medium for 24 h. a mRNA expression levels of alkaline phosphatase were measured by RT-Pcr. Data were analyzed using the 2-ΔΔCq method and normalized to β-actin. b Cell proteins were analyzed by Western blotting with antibodies specific to alkaline phosphatase and β-actin. c ALP activity was measured by pNPP method. Data are represented as mean ± SD. Statistical analysis: Student’s t-test vs THF 5 and 10 µM respectively, *p < 0.05; ***p < 0.001. THF tetrahydrofuran