Fig. 2
From: Lycopene and bone: an in vitro investigation and a pilot prospective clinical study
Lycopene decreases RANKL mRNA expression levels and increases COL1A mRNA levels on Saos-2 cells Semi-confluent cultures of human osteoblast-like cells (Saos-2) were incubated with lycopene 5 and 10 µM or vehicle THF in serum free medium for 24 h. mRNA expression levels of RANKL (a) and COL1A (b) were measured by RT-Pcr. Data were analyzed using the 2-ΔΔCq method and normalized to β-actin. Data are represented as mean ± SD. Statistical analysis: Student’s t-test vs THF 5 and 10 µM respectively, *p < 0.05. THF tetrahydrofuran, RANKL receptor activator of nuclear factor-kB ligand, COL1A type 1A collagen