Fig. 3

Influence of patient serum on endothelial nitric oxide synthase (eNOS) phosphorylation. a Human dermal microvascular endothelial cells (hdMVEC) were incubated with diluted serum samples collected from different patients at the various time points (baseline, after 5 L of fluid administration, and postoperatively), and eNOS phosphorylation was determined fluorometrically. b Graph shows association between individually increased serum ANG-2 levels and a reduction in eNOS phosphorylation ex vivo (Spearman rank correlation, dashed lines indicate the 95% confidence interval)