Skip to main content
Fig. 1 | Journal of Translational Medicine

Fig. 1

From: C-reactive protein derived from perivascular adipose tissue accelerates injury-induced neointimal hyperplasia

Fig. 1

Effect of HFD on the expression of CRP in PVAT and confirmation of CRP expression specifically in adipose tissue of CRPTG mice. a mRNA expression of CRP in PVAT was quantified by RT-PCR after WT C57BL6 mice received the HFD for 2 or 8 weeks. b Protein expression of CRP in PVAT was quantified by western blotting after mice were fed the HFD for 8 weeks; the values were normalized to β-tubulin expression as a control. c Gene expression of human CRP was detected by PCR and agarose gel electrophoresis based on genomic DNA extracted from tails of TG mice. d, e Protein expression of human CRP in perivascular adipose tissue (PVAT) was detected by western blotting and immunohistochemical staining. CON control, CRPTG CRP-overexpressing transgenic mice, huCRP human C-reactive protein. Scale bars, 100 μm. *P < 0.05 versus standard diet. Data represent mean ± standard error of the mean (SEM, n = 3)

Back to article page