Fig. 5

Silencing lncARSR alleviates lipid accumulation in HFD-fed mice and inhibits tumor formation in nude mice. HFD-fed mice were injected with lentivirus of sh-lncARSR or sh-NC. a LncARSR expression in mice detected by RT-qPCR. b Lipid accumulation in mice determined by H&E staining and Oil Red-O staining (×200). c TG content in mice. d IRS2 expression in mice detected by RT-qPCR. e Expression YAP1, IRS2 and AKT and phosphorylation of YAP1 and AKT levels detected by western blot analysis. f Expression of adipogenesis related proteins (Fasn, Scd1 and GPA) in mice detected by western blot analysis. HFD-fed mice were injected with HepG2 cells stably transfected with sh-lncARSR or sh-NC. g Tumor size and volume examined in xenograft tumor model. n = 15 *p < 0.05 against NAFLD mice injected with sh-NC lentivirus or sh-NC-transfected HepG2 cells. Differences between two groups were analyzed by unpaired t-test. Results were expressed as mean ± standard deviation