Whole transcriptional analysis identifies markers of B, T and plasma cell signaling pathways in the mesenteric adipose tissue associated with Crohn’s disease

Table 1 Clinical and demographic characteristics of the patients included in the analysis of RNA-seq, qPCR and immunostaining

	RNA sequencing and qPCR cohort			qPCR and IH cohort
	CD group	CTR group (MAT)	CTR group (ileal mucosa)	CD group	CTR group (MAT)	CTR group (ileal mucosa)
Number	8	4	4	18	13	8
Gender (M/F)	4/4	2/2	2/2	6/12	4/9	2/6
Age (years)	38 [20–66]	64.5 [58–67]^#	57.5 [44–60]	36 [20–70]	56 [26–61]	57 [44–71]^#
Disease duration (years)	6 [1–14]	–	–	6.5 [1–30]	–	–
Age at diagnosis (A1/A2/A3)^a	1/5/2	–	–	1/14/3	–	–
Location (L1/L2/L3/L4)^a	4/0/4/0	–	–	5/0/13/0	–	–
Behaviour (B1/B2/B3)^a	0/3/5	–	–	0/12/6	–	–
Perianal disease (yes/no)	1/7	–	–	2/16	–	–
Immunosuppressant (yes/no)	5/3	–	–	7/11	–	–
Anti-TNFα therapy (yes/no)	8/0	–	–	18/0	–	–
CDAI	301.9 [261.4–609.6]	–	–	325.5 [162–479.8]	–	–
Presence of ulcers (yes/no)	8/0^b	0/4^c	0/4^c	18/0^b	0/13^c	0/8^c
Body weight (kg)	58 [48–79]	60 [52–63]	65.8 [54–72.6]	56.5 [43.4–78]	74 [49–107]	72 [58–98]
Body mass index	22.7 [15.5–26.5]	21 [20.8–21.5]	26.8 [24–29.6]	21.4 [14.7–30.4]	29.7 [21.2–36.1]	25.5 [22.7–38]

Numerical variables are described as median [min, max] and categorical variables as absolute frequencies
CD Crohn’s disease, MAT mesenteric adipose tissue, M male, F female, TNF tumor necrosis factor, CDAI Crohn’s Disease Activity Index
^#p < 0.05 is considered statistically significant versus CD group
^aMontreal classification
^bPresence of ulcers in the ileum as evaluated by macroscopic and microscopic histological examination of the surgical specimen by a pathologist. The pathology reports by an experienced pathologist identified some degree of fibrostenotic disease in the surgical specimen together with the presence of ulcers and infiltrating immune cells in the mucosa of all patients included in the study
^cPresence of ulcers in the ileum as evaluated by colonoscopy

ISSN: 1479-5876