Fig. 4

Expression of angiogenesis-related genes. a The expression of VEGF-A was higher in the A-07 model than in the D-12 model, both in cultured cells and meningeal tumors. b The expression of 84 angiogenesis-related genes in the D-12 model versus that in the A-07 model in cultured cells and meningeal tumors. The lines indicate a fivefold difference in expression level. Of the 84 genes, 38% (cells) and 42% (tumors) showed more than fivefold higher expression in the D-12 model than in the A-07 model (red symbols), whereas 11% (cells) and 5% (tumors) showed more than fivefold higher expression in the A-07 model than in the D-12 model (green symbols). c The expression of the specific genes showing expression levels differing by a factor of more than 5 between meningeal A-07 and D-12 tumors. Gene expression was measured with quantitative PCR and normalized to the expression of 5 housekeeping genes. Columns and points: mean of 3 cell cultures or tumors. Bars: standard error. P-values: two-sided t-test