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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Selenium-binding protein 1 transcriptionally activates p21 expression via p53-independent mechanism and its frequent reduction associates with poor prognosis in bladder cancer

Fig. 3

Overexpression of SELENBP1 inhibits bladder cancer cell growth. a Cell lysates of human bladder cancer cell lines were extracted and subjected to immunoblotting assay for determining the levels of SELENBP1 protein expression. GAPDH was used as a loading control. b UMUC3 cells were stably transfected with empty vector (Vector) or construct encoding HA-tagged SELENBP1 (HA-SELENBP1). Cell lysates of these stable transfectants were extracted and subjected to immunoblotting assay by using anti-HA antibody. GAPDH was used as a loading control. c The number of 1 × 104 stable UMUC3 (Vector) and UMUC3 (HA-SELENBP1) cells per well was seeded in 6-well plates. The number of viable cells at the indicated time points was determined by trypan blue exclusion assay. The values were shown as mean ± standard deviation from at least three independent experiments. The asterisk (*) indicates a significant difference in comparison to that of control group (p < 0.05). d Anchorage-independent cell growth of UMUC3 cells stably transfected with empty vector or construct encoding HA-SELENBP1 was determined by soft agar assay. The colony formation was observed and captured under an inverted microscope (×40 magnification; left panel) and numbers of colonies were scored on day 15, and presented as colonies (×100)/104 cells (right panel). The values were shown as mean ± standard deviation from at least three independent experiments. The asterisk (*) indicates a significant difference in comparison to that of control transfectants (p < 0.05)

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