Table 1 Summary of antibodies/fluorochromes used in this study
Target cell type | Antigen | Fluorochrome | clone | Supplier (Cat No) | Amount (µl) | Isotype control |
|---|---|---|---|---|---|---|
T cell | CD3 | PECy7 | UCHT1 | EBioscience (25-0038-42) | 5 | Mouse IgG1 k-PECy7 |
Monocyte | CD14 | APC | 61D3 | EBioscience (17-0149-42) | 5 | Mouse IgG1 k-APC |
Neutrophil | CD15 | BV786 | HI98 | BD Biosciences (563838) | 2.5 | Mouse IgM-BV786 |
Monocyte/NK cell, Neutrophil | CD16 | BUV395 | Â 3G8 | BD Biosciences (563785) | 2.5 | Mouse IgG1 k-BUV395 |
B cell | CD19 | BV650 | SJ25C1 | BD Biosciences (563226) | 5 | Mouse IgG1 k-BV650 |
Platelet | CD42b | BV421 | HIP1 | BioLegend (303930) | 2.5 | Mouse IgG1 k-BV421 |
Leukocyte | CD45 | PerCP-cy5.5 | 2D1 | BD Biosciences (340953) | 30 | Mouse IgG1, k-PerCP-cy5.5 |
NK cell | CD56 | FITC | NCAM16.2 | BD Biosciences (340410) | 10 | Mouse IgG2b-FITC |
Eosinophil | CD193 | APCCy7 | 5E8 | BioLegend (310712) | 5 | Mouse IgG2b-APCCy7 |
Basophil | CD203c | PE | NP4D6 | BioLegend (324606) | 10 | Mouse IgG1 k-PE |
| Â | Â | Â | Â | Â | Total: 77.5 | Â |