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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Immune cell phenotyping in low blood volumes for assessment of cardiovascular disease risk, development, and progression: a pilot study

Fig. 3

Lymphocyte phenotyping. A Representative example of flow cytometry gating scheme to identify B cells (A, f), T cells (A, g), NKT cells (A, g), and NK cells (A, h). NK cells can further be sub-gated to allow identification of cytotoxic (CD56dim/CD16high) or proliferative NK cells (CD56high/CD16dim) (A, i). Each cell subsets CD42b (platelet)-positive population can also be identified (A, j–m). B Identified cell populations are presented in percent (%) of all CD45-positive cells displayed in (A, d). C Sub-gating of NK cells (A, h) by CD56 and CD16 allows for quantification of proliferative versus cytotoxic NK cell populations displayed as percent of CD3-/CD56+ NK cells (A, h). D Platelets adherent to each cell population are presented in percent (%) of originating gate. Representative quantitative results of 29 healthy adult blood donors. E–G Scanning electron micrographs displaying the indicated population and the adherent platelet. Adherent platelets are indicated by the red arrow. Data are represented as mean ± the standard error of the mean

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