Fig. 2

Monocyte phenotyping. A Representative example of flow cytometry gating scheme to identify monocytes and their subsets (A, f, g). Each cell subsets CD42b (platelet)-positive population can also be identified (A, h–k). (B) Identified cell populations are presented in percent (%) of all CD45-positive cells displayed in (A, d). C Platelets adherent to each cell population are presented in percent (%) of originating gate (A, f, g). D, E Scanning electron micrographs displaying the monocytes with adherent platelets. Adherent platelets are indicated by the red arrows and stay adherent on monocytes during the process of macrophage differentiation (E). Representative quantitative results of 29 healthy adult blood donors. Data are represented as mean ± the standard error of the mean. (NM-nonclassical monocytes, IM-intermediate monocytes, CM-classical monocytes HMDM-human monocyte-derived macrophages)