Fig. 3

tolDC induce functional IL-10-producing antigen-specific Tr1 cells. Cell proliferation dye (CFSE/CTV)-labelled PBMC of healthy controls were cultured with the recall antigen PPD and tolDC (tol), imDC (im) or without addition of any type of moDC (−) for 9 (a) or 6 (b) days. a Cytokine secretion in culture supernatants was measured using ELISA. Repeated measures ANOVA was used. b CFSE/CTV⁻ live CD4⁺ (total CD4⁺) and CD4⁺LAG3⁺CD49b⁺ T-cells (Tr1) were sorted, rested with 10 IU/ml of IL-2 for 2 days and restimulated by plate bound anti-CD3 (10 μg/ml) and soluble anti-CD28 (1 μg/ml) for 3 days. Cytokine secretion in culture supernatants was measured using MSD immunoassay. One-tailed paired Student T-test was used. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001