Fig. 6

Cell viability determination. Cell viability comparison between manual and automatic PBMC purification. The viability assay using 7AAD (7-ammino-actinomycin D) was performed on three blood samples after manual (a) and automatic isolation (b). The gating strategy foresees debris and doublets exclusion as describe in FCS-A vs SSC-A and FSC-H vs FSC-A dot-plot, respectively. The cellular population to be tested for 7-AAD staining was selected in terms of CD45 positive events as shown in the CD45-Krome Orange (KO-525) vs SSC-A dot-plot. Dead cells were determined as 7-AAD positive events highlighted in red in the 7-AAD vs SSC-A dot-plot. No significant differences were observed between the two methods (unpaired t-test) as regards the percentage of live cells that was > 98% in all cases