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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Purification of viable peripheral blood mononuclear cells for biobanking using a robotized liquid handling workstation

Fig. 2

Automatic workflow diagram. Workflow showing the automatic procedures performed by the Freedom EVO 150 for blood stratification and PBMC collection. The blood stratification procedure consists of four necessary steps: (1) density media dispensation; (2) blood sample dilution; (3) diluted blood stratification, and (4) tube recovery for centrifugation (step highlighted in yellow). PBMC collection was performed after centrifugation and consisted of two steps: (1) the removal of 1.5 mL of supernatant and (2) the collection of PBMCs by means of aspirating 3.4 mL of the cellular suspension and subsequent distribution in 15 mL conical tubes containing DPBS + 2% FBS

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