Skip to main content

Table 5 Expected quality for frozen tissue samples based on RNA quality assessment according to pre-analytical factors prioritized following SPREC and BRISQ recommendations

From: In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network

Type of codificationVariableOptimal expected qualityModerate expected qualitySub-optimal expected quality
BRISQAnatomical siteColon, lung and liverFatty
Fibrous
Pancreatic
Neuronal [74,75,76]
BRISQBody temperature4 °C (post-mortem) [77]RT 18–28 °C (post-mortem) [77]Unknown
37 °C (alive)
SPRECType of sampleCEN, CLN, FNA, LCM, PLC, TISHAR, PEN, TCMZZZ
SPRECType of collectionA06, BCM, BPS, BSL, BTM, FNA, PUN, SRG, SSL, STM, VACA12A24, A48, A72 [77]
SWB
SPRECWarm ischemia timeA, B, C, D, NEF, X
SPRECCold ischemia timeA, B, C, D [20, 78]E, NF, X [30, 66, 79]
SPRECFixation/stabilization typeOCT, PXT [80, 81]NoneOthers (ACA, ALD, FOR, HST, NAA, NBF, XXX, ZZZ) [83]
RNL [32, 82]
SNP [24, 80, 81]
ALL
SPRECFixation/stabilization timeD, E (PXT) [84]A, B, CX
F (ALL, RNL) [85]D, E (ALL, RNL) [85]
NF, G (PTX) [84]
 G (ALL, RNL) [85]
 X (ALL, PXT, RNL)
SPRECLong-term storageA, J, N Q, S, W [86]B, V, C, D, E, F, G, H, I, K, T, XP [27, 87]
Z
BRISQStorage duration< 5 years [88, 89]5–20 years [89]> 20 years