In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network

Table 5 Expected quality for frozen tissue samples based on RNA quality assessment according to pre-analytical factors prioritized following SPREC and BRISQ recommendations

Type of codification	Variable	Optimal expected quality	Moderate expected quality	Sub-optimal expected quality
BRISQ	Anatomical site	Colon, lung and liver	Fatty	–
			Fibrous
			Pancreatic
			Neuronal [74,75,76]
BRISQ	Body temperature	4 °C (post-mortem) [77]	RT 18–28 °C (post-mortem) [77]	Unknown
BRISQ	Body temperature	37 °C (alive)	RT 18–28 °C (post-mortem) [77]	Unknown
SPREC	Type of sample	CEN, CLN, FNA, LCM, PLC, TIS	HAR, PEN, TCM	ZZZ
SPREC	Type of collection	A06, BCM, BPS, BSL, BTM, FNA, PUN, SRG, SSL, STM, VAC	A12	A24, A48, A72 [77]
SPREC	Type of collection	A06, BCM, BPS, BSL, BTM, FNA, PUN, SRG, SSL, STM, VAC	A12	SWB
SPREC	Warm ischemia time	A, B, C, D, N	E	F, X
SPREC	Cold ischemia time	A, B, C, D [20, 78]	E, N	F, X [30, 66, 79]
SPREC	Fixation/stabilization type	OCT, PXT [80, 81]	None	Others (ACA, ALD, FOR, HST, NAA, NBF, XXX, ZZZ) [83]
		RNL [32, 82]
		SNP [24, 80, 81]
		ALL
SPREC	Fixation/stabilization time	D, E (PXT) [84]	A, B, C	X
		F (ALL, RNL) [85]	D, E (ALL, RNL) [85]
		N	F, G (PTX) [84]
			G (ALL, RNL) [85]
			X (ALL, PXT, RNL)
SPREC	Long-term storage	A, J, N Q, S, W [86]	B, V, C, D, E, F, G, H, I, K, T, X	P [27, 87]
SPREC	Long-term storage	A, J, N Q, S, W [86]	B, V, C, D, E, F, G, H, I, K, T, X	Z
BRISQ	Storage duration	< 5 years [88, 89]	5–20 years [89]	> 20 years

ISSN: 1479-5876