Skip to main content

Table 1 Summary of publications evaluating quality of RNA samples

From: In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network

Measurement methodAnalytical techniqueEvaluated parameterThresholdOrganReferences
SpectrophotometryUV spectroscopy (A260/280) NanoDropQuantity and purityAround 2Human trabecular bone[13,14,15,16,17,18]
> 1.8 excellentColon, articular cartilage and subchondral bone, brain
1.8–1.6 adequate
< 1.6 inadequate
UV spectroscopy (A260/230) NanoDropQuantity and purity> 2 non contaminated RNAArticular cartilage and subchondral bone[16]
< 2 contaminated RNA
ElectrophoresisRIN, RIS, or equivalentIntegrity≥ 7 high-integrity RNAColon, kidney, placenta, articular cartilage and subchondral bone, trabecular bone, pancreas[13, 14, 16, 19,20,21,22,23,24,25,26,27]
6–7 adequate-integrity RNATrabecular bone, pancreatic, stomach, liver, colon, brain[13, 26, 28,29,30,31,32,33]
5–6 low integrityPancreas, breast, thyroid, stomach, lung, colon[26, 34, 35]
3–5 partially degradedBreast, thyroid, stomach, lung, colon, kidney, pancreas[14, 26, 33,34,35]
1–3 totally degradedTrabecular bone, breast, thyroid, stomach, lung, colon, brain, placental[13, 17, 27, 34, 36]
DV200Integrity> 70% high qualityBrain and other tissue types[37, 38]
50–70% medium quality
30–50% low quality
< 30% too degraded
28S:18S peak ratioIntegrityAround 2Stomach, pancreas, liver, colorectal[29]
Electrophoretic profileIntegrity2 bands 2000 nt (18S), 4000 nt (28S) → (Non-degraded RNA)Pancreatic tissue[26, 27]
Diffuse banding indicative of degraded RNAPancreatic tissue[26, 27]
RT-qPCR3′:5′ ratioIntegrity1–5 perfectly intact mRNAN/A[39]
> 5 suggests degradation
≥ 10 denatured mRNA
Ct valuesFunctionalityIncreasing Ct values of ABL1, FOSB and JUN genes suggest RNA degradationColon[40]