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Table 1 Summary of publications evaluating quality of RNA samples

From: In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network

Measurement method Analytical technique Evaluated parameter Threshold Organ References
Spectrophotometry UV spectroscopy (A260/280) NanoDrop Quantity and purity Around 2 Human trabecular bone [13,14,15,16,17,18]
> 1.8 excellent Colon, articular cartilage and subchondral bone, brain
1.8–1.6 adequate
< 1.6 inadequate
UV spectroscopy (A260/230) NanoDrop Quantity and purity > 2 non contaminated RNA Articular cartilage and subchondral bone [16]
< 2 contaminated RNA
Electrophoresis RIN, RIS, or equivalent Integrity ≥ 7 high-integrity RNA Colon, kidney, placenta, articular cartilage and subchondral bone, trabecular bone, pancreas [13, 14, 16, 19,20,21,22,23,24,25,26,27]
6–7 adequate-integrity RNA Trabecular bone, pancreatic, stomach, liver, colon, brain [13, 26, 28,29,30,31,32,33]
5–6 low integrity Pancreas, breast, thyroid, stomach, lung, colon [26, 34, 35]
3–5 partially degraded Breast, thyroid, stomach, lung, colon, kidney, pancreas [14, 26, 33,34,35]
1–3 totally degraded Trabecular bone, breast, thyroid, stomach, lung, colon, brain, placental [13, 17, 27, 34, 36]
DV200 Integrity > 70% high quality Brain and other tissue types [37, 38]
50–70% medium quality
30–50% low quality
< 30% too degraded
28S:18S peak ratio Integrity Around 2 Stomach, pancreas, liver, colorectal [29]
Electrophoretic profile Integrity 2 bands 2000 nt (18S), 4000 nt (28S) → (Non-degraded RNA) Pancreatic tissue [26, 27]
Diffuse banding indicative of degraded RNA Pancreatic tissue [26, 27]
RT-qPCR 3′:5′ ratio Integrity 1–5 perfectly intact mRNA N/A [39]
> 5 suggests degradation
≥ 10 denatured mRNA
Ct values Functionality Increasing Ct values of ABL1, FOSB and JUN genes suggest RNA degradation Colon [40]