Fig. 5

HO-1 regulates EZH2 via the pRB-E2F pathway. a HO-1 expression was regulated by a lentivirus (HO-1) or small interfering RNA (siHO-1). EV1 and EV2 represent the corresponding empty carriers. MDS1-si-HO-1 indicates downregulation of HO-1 expression. MDS2-HO-1 indicates upregulation of HO-1 expression. We divided these MDS-2-HO-1 cells into a control group (Con) and 5 experimental groups. The control group was not treated with HLM006474. We used different concentrations of HLM006474 to treat the experimental group cells. b After regulating HO-1, the expression levels of downstream genes were very different. We used three rulers to represent the value. c We regulated HO-1 and detected apoptotic genes (caspase-3, caspase-9 and PARP) and cycle-related genes (CDK4 and CDK6). d Dec represents decitabine. P1-P4 represents four MDS patients. These four MDS patients had overexpressed HO-1. They were resistant to decitabine and progressed to AML. e The expression of proteins in the cells was detected by laser scanning confocal microscopy. DAPI was used for nuclear staining. Merge refers to the overlap image. We also used the Quantity one software to analyze gray values. The relevant values are shown in the histogram. Each sample was normalized to related β-actin expression. All the experiments were repeated, *P < 0.05 (statistically significant)