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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: HO-1 promotes resistance to an EZH2 inhibitor through the pRB-E2F pathway: correlation with the progression of myelodysplastic syndrome into acute myeloid leukemia

Fig. 3Fig. 3

MDS cells overexpressing EZH2 and HO-1 inhibit the expression of P15INK4B and TP53 in mice. a MDS mouse models were established with male C57BL/6Ly5.2 mice. Five mice were included in the control group and injected with only compete medium containing 15% fetal bovine serum for tumor cell culture. The other 5 mice were included in the experimental group. Tumor cells were injected via the tail vein. In the experimental group, per mice were injected with 3 × 107 MDS cell suspension. We injected the mice every day and measured body weight every 5 days. The weights of the mice were recorded. b We harvested the spleens from the control and experimental mice. Then we recorded spleen size and weight. c The spleen sections were evaluate by hematoxylin and eosin (H&E) staining. The infiltration of the tumor cells into various organs were observed. Magnification, ×400 or ×800. d Immunohistochemical (IHC) staining was used to evaluate organ sections. The images show positive reactions for HO-1, EZH2, pRB, E2F, P15INK4B (P15) and TP53 (P53). Magnification, ×400. Positive staining intensity was scored as 1. Cells that were not stained were scored as 0. Light brown cells that were weakly positive were scored as 1. Cells that were brown with no background staining or cells that were dark brown, but with a light brown background, (medium positive) were scored as 2. Cells that were dark brown with no background staining (strongly positive) were scored as 3. We showed the most representative images. All the experiments were repeated, *P < 0.05 (statistically significant)

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