Fig. 2

The effect of OLR1 in osteosarcoma cells. 2-1. The effect of OLR1 on colony formation assays in osteosarcoma cell lines. a Culturing equal numbers of OLR1-overexpressing U2-OS cells and vector controls for 48 h, much higher cell densities for the OLR1-overexpressing U2-OS cells were observed. b Culturing equal numbers of OLR1-silencing 143b cells and controls for 48 h, much lower cell densities for the OLR1-silencing 143b cells were observed. c Culturing equal numbers of OLR1-silencing MG63 cells and controls for 48 h, much lower cell densities for the OLR1-silencing MG63 cells were observed. 2-2. The effect of OLR1 on cell proliferations in osteosarcoma cell lines. a Cell proliferations of OLR1-overexpressing U2-OS cells were enhanced. b Cell proliferations of OLR1-silencing 143b cells were reduced. c Cell proliferations of OLR1-silencing MG63 cells were reduced. 2-3. The effect of OLR1 on cell cycle in osteosarcoma cells. a OLR1 overexpression triggered G1 to S + G2 phases of cell cycle in U2-OS cells. b OLR1-silencing blocked G1 to S + G2 phases of cell cycle in 143b cells. C: OLR1-silencing blocked G1 to S + G2 phases of cell cycle in MG63 cells. 2-4. The effect of OLR1 on migratory abilities in osteosarcoma cells. a The overexpression of OLR1 in U2-OS cells increased migratory abilities. b 143b cells migratory abilities were suppressed when OLR1 was silenced. c MG63 cells migratory abilities were suppressed when OLR1 was silenced. 2-5. The effect of OLR1 on invasive abilities in osteosarcoma cells. a The overexpression of OLR1 in U2-OS cells increased invasive abilities. b 143b cells invasive abilities were suppressed when OLR1 was silenced. c MG63 cells invasive abilities were suppressed when OLR1 was silenced