Fig. 1

The expression levels of OLR1 in osteosarcoma. 1-1. Real-time PCR analysis to screen for activated metastasis-driving genes in osteosarcoma. Heatmap clustering of expression array data obtained from 12 pairs of primary and metastatic tumors tissues. 1-2. Real-time PCR analysis to screen for activated metastasis-driving genes in osteosarcoma. Heatmap clustering of expression array data obtained from 31 pairs of primary and metastatic tumors tissues. 1-3. The expression levels of OLR1 in osteosarcoma tissues. a The expression levels of OLR1 in 31 pairs of human primary osteosarcoma tissues and matched metastatic osteosarcoma tissues. The expression level of OLR1 was significantly upregulated in metastatic OS tissues in comparison to the primary OS tissues (P = 0.004). b The expression levels of OLR1 in 30 pairs of human primary osteosarcoma tissues from metastatic and non-metastatic osteosarcoma patients. Higher OLR1 expression level was observed in the primary osteosarcoma tissues from metastatic patients (P < 0.001). 1-4. The expressions of OLR1 in osteosarcoma cell lines. a It was shown by qRT-PCR that the expressions of OLR1 were low in U2-OS and SAOS-2 cell lines, but high in 143b and MG63 cell lines. b It was validated by Western blotting that the expressions of OLR1 were low in U2-OS and SAOS-2 cell lines, but high in 143b and MG63 cell lines. 1-5. The expressions of OLR1 in osteosarcoma cell lines after relevant treatment. a After the full-length OLR1 gene was overexpressed in U2-OS cells, a high expression was achieved. b Lentiviral constructs encoding OLR1-targeting shRNAs were transferred into MG63 and 143b cells. The expression levels of OLR1 were validated by Western blotting