Fig. 3

Fracture-induced c-Fos expression is reduced in the superficial layers of the spinal cord dorsal horn in B2 receptor knockout mice (B2KO). c-Fos expression was assessed by immunohistochemistry at the L3–L5 level 120 min after fracture induction. a–d Are photomicrographs of c-Fos labeling from representative sections obtained from sham wild-type (WT) (a), fractured WT (b), fractured B1 receptor knockout mice B1KO (c) and fractured B2KO (d) mice, ×20 objective. Images are zoomed on layers 1 and 2 were most of the c-Fos labelling is observed. In (e), bar graph illustrating the mean ± SEM of the number of c-Fos positive nuclei/section from six animals in each group. **P < 0.01, ***P < 0.001 compared to ipsilateral sham WT. One-way ANOVA followed by Bonferroni’s post-hoc test. ^#P < 0.05 compared to ipsilateral fractured WT