Fig. 1

Mechanical (a), thermal (b) hyperalgesia and subjective pain (c) caused by fractured tibia are reduced in B1 and B2 receptor knockout (B1KO, B2KO) compared to wild-type (WT) mice. After baseline testing, male adult mice were subjected to closed tibial fracture and tested for paw withdrawal threshold to evaluate mechanical sensitivity (a) or paw withdrawal latency to evaluate thermal sensitivity (b). Subjective pain (c) was expressed by a rating scale from 1 to 5 as described in “Materials and methods” section. The contralateral side was also tested at each time point for each group of mice: the mechanical thresholds were 8 g (cut off), the heat latencies were 12 s (cut off), and the subjective pain scores were zero in all groups at all time points. Each bar represents mean ± SEM of 9 mice per group. Data were subjected to Friedman’s test followed by Wilcoxon’s signed rank test. *P < 0.05 when comparing B1KO to WT. ^#P < 0.05 when comparing B2K0 to WT