Fig. 3

Exogenous miRNA-21 inhibits CVB3 replication by targeting MAP2K3 in HeLa cells. HeLa cell were transduced with Lenti-miRNA-21 at MOI = 10, and were then infected with CVB3 at MOI = 1 3 days later (miR21-up as shown in the figure). Cells transduced with Lenti-CON followed by CVB3 infection were used as control group (miR-con as shown in the figure). a miRNA-21 expression in HeLa cells after transduction with Lenti-miRNA-21 or control lentivirus 3 days and 5 days later, miR-con represents cells transduced with Lenti-CON and miR21-up represents cells transduced with Lenti-miRNA-21. b Western blotting was conducted to detect MAP2K3, P-P38 MAPK and P-HSP27 expression in HeLa cells 7 h post CVB3 infection in Lenti-miRNA-21 transduced cells. GAPDH was loaded as a control group. Shown were representative results from 3 independent test. c CVB3 replication in the supernatants post CVB3 infection at indicated time point in Lenti-miRNA-21 transduced cells. Virus titer values represent the means and standard deviations of three independent experiments, (*p < 0.05)