|
Unigene accession no.
|
Gene ID
|
Gene
|
Left primer
|
Right primer
|
Amplicon length (bp)
|
|---|
|
Hs.171189
|
196
|
AHR
|
CTTCCAAGCGGCATAGAGAC
|
AGTTATCCTGGCCTCCGTTT
|
198
|
|
Hs.274402
|
3303
|
HSPA1Aa,b
|
CCCTGATCAAGCGCAACT
|
CTCGTACACCTGGATCAGCA
|
98
|
|
Hs.654458
|
3569
|
IL6
|
CAATGAGGAGACTTGCCTGGT
|
AGCTGCGCAGAATGAGATGA
|
275
|
|
Hs.193717
|
3586
|
IL10
|
GGCGCTGTCATCGATTTCTTC
|
GCCACCCTGATGTCTCAGTT
|
181
|
|
Hs.126256
|
3553
|
IL1 beta
|
AGCCATGGCAGAAGTACCTG
|
CCTGGAAGGAGCACTTCATCT
|
116
|
|
Hs.673
|
3592
|
IL12A
|
GCTCCAGAAGGCCAGACAAA
|
GCCAGGCAACTCCCATTAGT
|
183
|
|
Hs.201978
|
5742
|
PTGS1b
|
AGCTCGTAGGAGAGAAGGAGAT
|
AGTGTGGCCGTCTTGACAAT
|
253
|
|
Hs.196384
|
5743
|
PTGS2b
|
CAAATTGCTGGCAGGGTTGC
|
AGGGCTTCAGCATAAAGCGT
|
139
|
|
Hs.432121
|
7001
|
PRDX2
|
ACGAGCATGGGGAAGTTTGT
|
GCCTTTCCTGGGTCAGCATA
|
201
|
|
Hs.502823
|
25824
|
PRDX5
|
TCTTTGGGAATCGACGTCTC
|
ATTGCAGAAATCTGGCCAAC
|
229
|
|
Hs.463059
|
6774
|
STAT3
|
CGGAGAAGCATCGTGAGTGA
|
CTCTTCCAGTCAGCCAGCTC
|
95
|
|
Hs.241570
|
7124
|
TNF-alpha
|
CCCCAGGGACCTCTCTCTAA
|
TCTCAGCTCCACGCCATT
|
172
|
|
Hs.520640
|
60
|
Actin-betac
|
TGTGGCATCCACGAAACTAC
|
TGATCTTGATCTTCATTGTGCT
|
175
|
|
Hs.544577
|
2597
|
GAPDHc
|
GGCCTCCAAGGAGTAAGACC
|
CTGTGAGGAGGGGAGATTCA
|
130
|
- aThese primers recognize also HSPA1B, a minor isoform of HSPA1A
- bHSPA1A, protein name HSP70, inducible form; PTGS1 and PTGS2, protein name COX1 and COX2 (CICLOOXYGENASE 1 AND 2)
- cActin-beta and GADPH were the housekeeping genes utilized in the gene expression study
