Fig. 4

Proliferation of 293T miRNA-148a knockdown cells incubated with MDEs. 293T cells were transduced with lentivirus encoding miRNA-148a-specific shRNA (148 (−)) or a scrambled sequence (CONT). After selection, cells were analyzed for miRNA148a expression by fluorescence microscopy (a) and by qRT-PCR. qRT-PCR results were calculated by the 2^(− ∆∆CT) method, and values were normalized against RNU6B (b). MiRNA-148a expression following incubation of miRNA-148 (−) cells with MDEs (c). Cell proliferation of cells grown in 10% FCS (10%) or 0% FCS (0%) and incubated with MDEs (MIRZIP 148A 0% +exo) or without (MIRZIP CONT 0% +exo) was examined by methylene blue staining (d)