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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Milk-derived exosomes (MDEs) have a different biological effect on normal fetal colon epithelial cells compared to colon tumor cells in a miRNA-dependent manner

Fig. 1

Isolation of milk-derived exosomes (MDEs) and their uptake by different tumor and normal colon cells. Protein expression of CD81 as a marker of exosomes and HSP70 as a negative control in MDEs (a). MDEs were analyzed by transmission electron microscopy using negative staining (b). Particle size distribution (by intensity) of isolated MDEs determined by DLS (c). Labeled milk exosomes isolated from human milk were incubated for 4 h with normal intestinal (CCD 841) or colon cancer (LS123) cells. Images were obtained by fluorescence microscope analysis (d, e). MiRNA-148a expression in CCD 841 and LS123 cells incubated with (EXO) or without (CONT) MDEs was analyzed by qRT-PCR (f, g). qRT-PCR results were calculated using the 2^(− ∆∆CT) method, and values were normalized against RNU6, *p < 0.05 t test

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