Fig. 1From: Direct S-Poly(T) Plus assay in quantification of microRNAs without RNA extraction and its implications in colorectal cancer biomarker studiesDirect S-Poly(T) Plus assay in microRNA detection. a Procedure of directly analyzing circulating miRNAs; b effects of different buffers and proteinase K in the Direct S-Poly(T) Plus assay; c effects of different proportions of crude RNA in Poly(A)/RT reaction; d sensitivity of Direct S-Poly(T) Plus when the polyadenylation and reverse transcription were performed separately (two-step) or in a single reaction (one-reaction)Back to article page