Fig. 3

Effects of ERs on key members of Notch1 and GSK3β/β-Catenin pathways. a, b PC9/G and H1299 cells were transfected with si-NC or si-ERs for 48 h. The relative expression levels of Notch1, NICD, Hes1, β-Catenin, GSK3β and pGSK3β in PC9/G (a) and H1299 cells (b) were analyzed by western blot. c–e PC9/G cells were treated with different concentrations of 17β-E2 (E2) and Fulvestrant (Ful) for 12 h. The relative expression levels of ERα and ERβ (c), Notch1, NICD and Hes1 (d), β-Catenin and GSK3β (e) were analyzed by western blot. All experiments were repeated at least three times