Fig. 3
From: Medulloblastoma rendered susceptible to NK-cell attack by TGFβ neutralization
UCB-derived NK genetically modified to express TGF-β dominant negative receptor (TGF-β DNRII) can protect against medulloblastoma mediated immune suppression. a Cytotoxicity of untransduced (gray lines; 21.21 ± 1.19% killing at E:T 5:1 in the absence vs. 14.98 ± 2.11% in the presence of medulloblastoma-conditioned media, n = 8, p = 0.02) and transduced (black lines; 21.11 ± 1.84% killing at E:T 5:1 in the absence vs. 21.81 ± 3.37 in the presence of medulloblastoma-conditioned media, n = 8, p = 0.85) against Daoy cells. Dotted lines represent cells grown in the presence of medulloblastoma-conditioned media. b Example flow for a paired transduced and non-transduced NK cell line showing the effects of medulloblastoma-conditioned media on the expression of wildtype TGF-βRII. c Mean fluorescence intensity of TGF-β RII in untransduced (5697 ± 576 from 8554 ± 898 TGF-βRII MFI, n = 9, p = 0.0039) and transduced (73,827 ± 40,154 and 88,750 ± 64,061 TGF-βRII MFI, n = 9, p = ns) cells, in the presence and absence of medulloblastoma-conditioned media. d Example flow for a paired transduced and non-transduced NK cell line showing the effects of medulloblastoma-conditioned media on the expression of CD16. e Summary MFI for CD16 differences between transduced (mean increase 66,815 CD16 MFI, range − 275,307 to 114,000; n = 7, p = 0.0469) and nontransduced (mean decrease 63,395 CD16 MFI, range − 181,245 to 480,980) cells in the presence and absence of medulloblastoma-conditioned media. Negative values refer to increase in expression. Error bars refer to standard error of the mean