Fig. 3

Adipose tissue macrophages were reduced and unable to form CLS in TREM2^−/− mice under HFD. WT and TREM2^−/− mice of C57BL/6 of 6 weeks were fed with CFD or HFD for 12 weeks. a Representative images of F4/80 staining of epididymal adipose tissue of WT and TREM2^−/− mice after 12 weeks of HFD feeding. Original magnification is ×100 and ×400 (within box at bottom right), scale bar = 200 μm. b Representative images of FCM analysis of macrophages of WT and TREM2^−/− mice after 12 weeks of HFD feeding. c Macrophage proportion and d macrophage count of epididymal adipose tissue of WT and TREM2^−/− mice after 12 weeks of HFD feeding (N = 8/group). e Representative images of FCM analysis of macrophage phenotypes of WT and TREM2^−/− mice after 12 weeks of HFD feeding. Proportion of F4/80⁺CD11c⁺ (f) and F4/80⁺CD206⁺ (g) macrophages of WT and TREM2^−/− mice after 12 weeks of HFD feeding (N = 9/group). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001