Fig. 2

TREM2^−/− mice demonstrated greater adipocyte hypertrophy and adipocyte death under HFD. WT and TREM2^−/− mice of C57BL/6 of 6 weeks were fed with HFD for 12 weeks. a Mean cross sectional area of epididymal adipocytes of WT and TREM2^−/− mice after 12 weeks of HFD feeding (N = 394 from WT mice, N = 389 from TREM2^−/− mice). b Histogram of cross sectional area of epididymal adipocytes of WT and TREM2^−/− mice after 12 weeks of HFD feeding. c Representative images of perilipin staining of epididymal adipose tissue of WT and TREM2^−/− mice after 12 weeks of HFD feeding. Original magnification is ×100 and ×400 (within box at bottom right), scale bar = 200 μm. d Dead adipocytes proportion of WT and TREM2^−/− mice after 12 weeks of HFD feeding (N = 6/group). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001